Isolation and Antimicrobial Assessment of Crude Extract fromAspergillus sp. SO12 Isolated froma Marine Source

Document Type : Original Article

Authors

1 Microbial Chemistry Department, National Research Centre, El-Buhouth St. 33, Cairo 12622, Egypt

2 Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt

3 Microbiology Department, Central Lab. of Residue Analysis of Pesticides &Heavy Metals inFood

Abstract

The marine environment represents a rich source of microorganisms that havebeen increasingly explored for their bioactive potential. In this study, we report the isolationof Aspergillus sp. SO12 from a marine source and the subsequent extraction of its crudeextract. The bioactive potential of this extract was evaluated through antibacterial assaysagainst various bacterial strains and antifungal assessments against Candida albicans andAspergillus niger. The isolated strain, Aspergillus sp. SO12, was obtained froma marinesediment sample. Molecular characterization confirmed its identity as a member of theAspergillus genus, and subsequent cultivation allowed for the production of a crude extract. The extract was obtained using organic solvent. Antibacterial activity of the crude extract wasassessed against a panel of clinically relevant bacterial strains, including Gram-positive(Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli andPseudomonas aeruginosa) bacteria. The extract demonstrated significant antibacterial activity, with notable inhibition zones and minimum inhibitory concentrations (MICs), suggesting the
presence of bioactive compounds with potential therapeutic value against these pathogens. Furthermore, the antifungal potential of the crude extract was evaluated against Candidaalbicans, a common pathogenic yeast, and Aspergillus niger, a close relative to the producingfungus. The extract displayed potent antifungal activity against both Candida albicans andAspergillus niger, indicating its capacity to combat fungal infections. In summary, Aspergillus sp. SO12, isolated from a marine source, produces a crude extract withremarkable antibacterial and antifungal properties. The study underscores the potential of
marine-derived microorganisms in yielding bioactive compounds with applications inantimicrobial drug development and biotechnology. This research contributes toour
understanding of the bioactive potential of marine fungi and suggests promising avenues for
future investigations into novel antimicrobial agents.

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