Spectrophotometric determination of Tartrazine in soft drink powder and pharmaceutical dosage form

Ali, Ayman A.; Hassan, Abeer M.E.; Hussien, M.A.; Amin, Alaa S.; Abdelsalam, Ahmed M.

doi:10.21608/jbes.2020.371465

Spectrophotometric determination of Tartrazine in soft drink powder and pharmaceutical dosage form

Document Type : Original Article

Authors

¹ Chemistry Department, Faculty of Science, Benha University, Benha, Egypt

² Chemistry Department, Faculty of Pharmacy, October 6th University, October, Egypt

³ Biochemistry Department, Faculty of Pharmacy, October 6th University, October, Egyp

10.21608/jbes.2020.371465

Abstract

Simple and rapid spectrophotometric methods were utilized for the determination of tartrazine (E-102) (TZ) in soft
drink powder and pharmaceutical dosage forms. A study of the absorption spectra of tartrazine coloring agent has been
carried out by studying various parameters such as the effect of pH, type, and volume of surfactant and time. The
optimum analytical parameters and their effects are investigated. Beer’s law is obeyed in the range 2.0–20 µg mL-1 at λmax
= 427 nm. For more accurate analysis, Ringbom optimum concentration range is found to be 5-19 µg mL-1
. The molar
absorptivity and Sandell sensitivity were determined. The suggested method was successfully applied to the
determination of the examined coloring agent in soft drink powder and pharmaceutical dosage form.

Keywords