Studies on Methicillin-Resistant Staphylococcus aureus (MRSA) Infection Resistance for Antibiotic Using Nanoparticles and Essential Oil

Document Type : Original Article

Authors

1 Department of Microbiology Organization Benha University Address Benha, Egypt

2 Department of Tropical, Faculty of Medicine, Tanta University

3 R&D Department of Egyptian company for blood transfusion services (EGy BLOOD-VACSERA)

4 Assistant Professor of Molecular biololgy and Microbiology, Department of Botany and Microbiology, Faculty of Science, Benha University, Benha, Egypt

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a strain of S. aureus that has
developed resistance to nearly all β-lactam antibiotics, posing serious treatment challenges. Recent studies
have shown that metal oxide nanoparticles, particularly copper oxide (CuO), possess strong antimicrobial
properties against various pathogens, including S. aureus. Additionally, rosemary and its essential oil are
commonly used in the food industry due to their pleasant flavor and potent antioxidant effects. Therefore,
this study aims to improve the effectiveness of antibiotic treatments for MRSA infections through the use
of copper oxide nanoparticles in combination with natural rosemary oil.
Patients: This study was conducted on fifty blood samples collected from Patients infected with methicillin
resistant Staphylococcus aureus (MRSA) were obtained from clinical specimens. Patient samples will be
recruited from ICUs, Tanta University hospital, from October 2020 to May 2023.
Methods: The samples were inoculated into blood agar and MacConkey agar, then incubated both
aerobically at 37°c for 24hours.
Results: various combinations to nanoparticles and essential oil showed an enhanced activity to different
antibiotic at incubation for 24 h compared to antibiotics individually. Where, the combination of copper
oxide 50% concentration with Rosemary essential oil 60% concentration (inhibition zone mean 15.14
mm) showed improved to inhibition zone for different antibiotics to MRSA strains.
Conclusions: copper oxide nanoparticles have good effect for MRSA at high concentration. Also, zinc
oxide nanoparticles showed good effect at low concentration but less than copper oxide nanoparticles .

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