Microbial Interdisciplinary Experiment: Unravelling the outcome of Lglutaminase Produced by Streptomycetes isolates

Abdelmonem, Mohamed O.; El Awady, Mohamed E.; El-khonezy, Mohamed I.; Hassan, Mervat G.; Kishk, Mona A.

doi:10.21608/jbes.2023.372264

Microbial Interdisciplinary Experiment: Unravelling the outcome of Lglutaminase Produced by Streptomycetes isolates

Document Type : Original Article

Authors

¹ Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

² Microbial Biotechnology Department, Biotechnology Research Institute, National Research Centre, El-Buhouth St. 33, Dokki, Cairo, Egypt.

³ Molecular Biology Department, National Research Centre, 33-El Bohouth St., Dokki, Giza, P.O.12622, Egypt.

10.21608/jbes.2023.372264

Abstract

It is well known that Streptomyces produces beneficial pharmaceuticals, particularly antibiotics
and anticancer agents. This investigation uncovered 34 randomly selected streptomycetes isolates
in sediment, marine water, soil, and other habitats. Using a qualitative rapid plate assay to test for
GluNase synthesis, 9 of them produced a significant amount of L-glutamine pink colour.The
maximum levels of GluNase synthesis were obtained at a pH of 7.5, an incubation temperature
of 40 °C, and the utilization of glucose and peptone as the carbon and nitrogen sources,
respectively.

Keywords