Effect of cisplatin on chromosomes of male mice (Mus musculus)

Document Type : Original Article

Authors

1 Zoology Department, Faculty of Science, Benha University, Egypt

2 Zoology Department, Faculty of Science, Benha University, Egyp

Abstract

Cisplatin is an anticancer drug. It is used to treatment of cancer. The present work studies the effect of cisplatin on
chromosomes of male mice. 35 healthy adult male Swiss albino mice (Mus musculus) were divided into 7 groups,
composed of 5 animals each.
The first group was normal (control), the second, third and fourth groups were administered single doses of 5, 10 & 15
mg/kg of cisplatin I.P, respectively. Mice were then anesthetized and killed after one day of cisplatin administration. The
fifth, sixth and seventh groups were injected the same doses, but mice were anesthetized and killed after 5 days of
cisplatin injection. Bone marrow of mice femurs were prepared the examining of chromosomal aberrations and mitotic
index.
various chromosomal aberrations were recorded as structural aberrations (deletion, fragmentation, centric fusion, ring,
end to end, break and gaps) and as numerical aberration (monosomy and trisomy). Also, the result of this study revealed
that cisplatin caused reduction in the mitotic index at various doses. It was found that cisplatin had adverse effect on
chromosomes and mitotic index

Keywords

Journal of Basic and Environmental Sciences
Volume 5, Issue 4
October 2018
Pages 325-332

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