Prevalence of the beta-lactamase producing bacteria in diabetic foot infection of the Egyptian diabetic patients

Lela, Abdelgyed; Radwan, Ahmed; Fouda, Mohamed; Shash, Seham; Hazaa, Mahmoud

doi:10.21608/jbes.2017.369646

Prevalence of the beta-lactamase producing bacteria in diabetic foot infection of the Egyptian diabetic patients

Document Type : Original Article

Authors

¹ SpecialistMicrobiology at Elresala Lab.

² Genetics and Cytology department, National research Center(NRC), Giza, Egypt.

³ 3Assistant professor of Microbiology and immunology at liver and digestive surgery center at the Faculty of Medicine Mansoura University.

⁴ Professor of microbiology at faculty of science Banha University.

⁵ Professor of microbiology at faculty of science banha university

10.21608/jbes.2017.369646

Abstract

Adiabatic foot infection is one of the most feared complications of Diabetes mellitus. Many studies have reported on
the bacteriology of Diabetic Foot Infections (DFIs) over the past 25 years, but the results have been varied and often
contradictory. Determination the prevalence of beta-lactamase producing bacteria in diabetic foot infections and study
the pattern of antibiotic resistance to these isolates, which were received from Mansoura university Hospital by means of
antimicrobial susceptibility tests and phenotypic methods. Then confirmed by using Polymerase chain reaction (PCR) to
detect Extended-spectrum beta-lactamases (ESBL) genes encodingresistance to beta-lactam antibiotics.
A total of 59 bacterial isolates were obtained from 56 patients with diabetic foot ulcers. The age group of these patients
ranged from 40 to 70 years. Gram-positive cocci were more prevalent (50.8%) than gram-negative bacilli (49.15%). The
commonest isolate was Staphylococcus.aureus (33.9%). followed by 15.25 % Pseudomonas.aeruginosa, 11.86
%Proteus.mirabilis. The antibiotic sensitivity profiles of theisolated bacteria ,showed that most isolates were resistant to
different beta lactams antibiotics .The application of Polymerase chain reaction (PCR) showed that Extended-spectrum
beta-lactamases ESBL) producers and the blaTEM, blaSHV, blaCTX-M and blaOXA genes were detected.