Molecular Identification of Strawberry Latent Ring Spot Virus (SLRSV) In Egypt

EL-Morsy, Sh. I.; EL-Sheikh, M. A.; Abd El-Razik, R.A.; Youssef, Sahar A.; Shalaby, A.A.

doi:10.21608/jbes.2017.369638

Molecular Identification of Strawberry Latent Ring Spot Virus (SLRSV) In Egypt

Document Type : Original Article

Authors

¹ Agric. Botany-Genetics Dept., Fac. of Agric., Al-Azhar Univ. Cairo, Egypt

² Agric. Botany-Genetics Dept., Fac. of Agric., Al-Azhar Univ. Cairo, Egypt.

³ Virus and Phytoplasma Res. Dept., Plant Pathol. Res. Inst., Agric. Res. Centre, Giza, Egypt

⁴ Virus and Phytoplasma Res. Dept., Plant Pathol. Res. Inst., Agric. Res. Centre, Giza, Egypt.

10.21608/jbes.2017.369638

Abstract

Strawberry latent ringspot virus (SLRSV) isolated from symptoms and symptomless strawberry plants and
identified with a specific antiserum using Double Antibody Sandwich ELISA (DAS-ELISA). Survey was conducted
through three successive seasons during 2013-20l6 on commercial strawberry fields in four governorates in Egypt. The
percentages of infection which recorded from the survey were 7.5, 6.3, 5.9% (Al-Dair-region) and 9.4, 10.6, 10.5% (Kafer
Al-Shapy-region) in El-Qalubia governorate. In El-Behera governorate the percentage was 5.3, 6.0, 5.7% (Badrregion) and
4.0, 4.4, 5.2% (Al-Nobaria- region). El- Monufyia (Quesna-region) recorded 8.8, 8.9, and 9.2 %. Ismailia (fayed-region)
was 4.7, 5.0, and 5.5% respectively. SLRSV mechanically transmitted from infected strawberry plants onto 16 host species
belonging to seven families. Mottling, local lesion, leaf deformation, systemic and dwarf plants were appeared two weeks'
post inoculation on indicator host Chenopodium quinoa and Chenopodium amaranticolor. Reverse transcription
polymerase chain reaction (RT-PCR) was used to amplify 497 bp fragment using specific primers for the viral coat protein
gene, as a tool for molecular diagnosis. The amplified PCR fragments were cloned, sequenced and compared with other
of those sequences available in GenBank. Results observed that, the Egyptian SLRSV isolate was A+T, G+C (251, 246)
identical content with Poland and USA isolates. Obtained sequence comparison with the isolates available in the GenBank
indicated that SLRSV Egyptian isolate shared 99% identity with Poland isolate followed by 84% USA isolate, 83% UK
isolate and 80% Newzeland isolate.

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