Response of pathogenic bacteria isolated from wound infections to Antibiotics and some Essential oils from surgery department at Benha university hospital

Document Type : Original Article

Authors

1 B. sc. Microbiology and chemistry, faculty of science, Benha University (2008)

2 Lecturer of Microbiology & Immunology, Microbiology Department, Faculty of HumanMedicine, Benha University

3 Professor of Microbiology, Botany (Microbiology) Department, Faculty of Science, Benha University

Abstract

A total of 53 specimens were obtained from patients suffering from
wound infections in surgery department at Benha university hospital in
Qaliubiya province. The bacterial isolates identified as citrobacter
freundii (41.02%), staph aureus (23.07%), pseudomonas aeruginosa
(17.9%), coagulase negative staph (12.8%) and Klebsiella pneumonia
(5.12%).
The most effective antibiotics were ciprofloxacin, Amikacin and
Gentamycin against all gram negative bacteria, in case of gram
positive bacteria, the most effective antibiotics were Vancomycin
against staph aureus and Clindamycin against coagulase negative
staph. Beta lactam (Ampicillin, Amoxicillin clavulanate and cefoxitin)
were the most resistant antibiotics against Gram negative and Gram
positive bacteria.
Essential oils have more antimicrobial effect against gram positive
bacteria than gram negative bacteria.
Combination between antibiotics and essential oils were more effective
against different bacterial isolates than using both individually.
All beta lactamase producing strains of Klebsiella pneumonia and co
agulase negative staph (100%), (87.5%) of Citrobacter freundii, (80%) of pseudomonas aeruginosa and (85.7%) of staph aureus gave
positive result by the iodometric method.

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