Reveal of Antimicrobial Effect of Lawsonia inermis Extract on Candida albicans Using Molecular and Biochemical Techniques

Document Type : Original Article

Authors

1 Chemistry Department, Faculty of Science, Helwan University, Egypt

2 Chemistry Department, Faculty of Science, Helwan University, Egypt.

Abstract

Leaf samples of Lawsonia inermis(Li) was examined to their 
antimicrobial potential. Broth extracts in different concentrations 
were prepared and bioassayed in vitro for the growth of Candida 
albicans. The growth of Candida albicans pathogen was decreased.
In addition to the observed alterations which detected 
electrophoretically in the protein pattern and activity of the catalase 
enzyme. The protein pattern has one common band of Rf 0.28 and 
one characteristic band of Rf 0.48 for Candida albicans sample. The 
obvious quantitative mutation was observed in the fungi with 5, 10, 
15% of Lawsonia inermis compared with the control. Some types of 
proteins in Candida albicans were completely disappeared after 
inoculated with 5, 10, 15% Li. The catalase pattern showed one 
common band at R1 with Rf 0.067 and the characteristic bands observed 
at R5 with Rf 0.42 and at R6 with Rf 0.47 for Candida albicans 
inoculated with broth. These results confirmed the antifungal 
activity of henna leaves and supported the traditional use of the 
plant in therapy of bacterial infections. Disturbances occurred at the 
biochemical level. The broth extract of the Lawsonia inermis leaves 
showed obvious antifungal activity against Candida albicans. 

Keywords

Files

Share

How to cite

Statistics