Reveal of Antimicrobial Effect of Lawsonia inermis Extract on Staphylococcus aureus Using Molecular and Biochemical Techniques

Leaf of Lawsonia inermis (Li) was examined to their antimicrobial
potential. Broth extracts in different concentrations were prepared and
bioassayed in vitro for the growth of Staphylococcus aureus. It was found
that the growth of Staphylococcus aureus pathogen was decreased. The
protein pattern has one common band of Rf 0.24 and one characteristic
band of Rf 0.27 for Staphylococcus aureus sample. Small quantitative
mutation was observed in the bacteria with 5, 10, 15% of Lawsonia inermis
compared with the control. Some types of proteins in Staphylococcus
aureus were completely disappeared after inoculated with 5, 10, 15% Li.
The catalase pattern showed one common band at R1 with Rf 0.11 and the
characteristic bands observed at R2 , R3 , R4 , R5 with Rf ranged between
0.22- 0.41 for Staphylococcus aureus sample. These results confirmed the
antibacterial activity of henna leaves and supported the traditional use of
the plant in therapy of bacterial infections. The broth extract of the
Lawsonia inermis leaves showed obvious antibacterial activity against
Staphylococcus aureus.